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Nicola Casali : E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)
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Author: Nicola Casali
Title: E. coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)
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Published in: English
Binding: Hardcover
Pages: 316
Date: 2003-07-03
ISBN: 1588291510
Publisher: Humana Press
Weight: 1.41 pounds
Size: 6.1 x 0.87 x 9.17 inches
Edition: 2003
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Description: Product Description
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
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